Mysterious Microbe Plays Important Role in Ocean Ecology: Leads scientists to rethink ocean's carbon and nitrogen cycles

An unusual microorganism discovered in the open ocean may force scientists to rethink their understanding of how carbon and nitrogen cycle through ocean ecosystems.

A paper describing the new findings appears in the November 14 issue of the journal Science.

Two cyanobacteria fluoresce brightly inside a diatom seen under a microscope. Photo by Rachel Foster, University of California, Santa Cruz (UCSC)

 A research team led by Jonathan Zehr, a marine scientist at the University of California, Santa Cruz, (UCSC) characterized the new microbe by analyzing its genetic material, even though researchers have not been able to grow it in the laboratory.

Zehr said the newly described organism seems to be an atypical member of the cyanobacteria, a group of photosynthetic bacteria formerly known as blue-green algae.

"This research has revealed a big surprise about the microbiology of the oceans, and the complex integration of the ocean's nitrogen and carbon cycles," said Phillip Taylor, section head in the National Science Foundation (NSF)'s Division of Ocean Sciences, which funded the work.

"The fact that nitrogen fixation in these abundant unicells is decoupled from photosynthesis is intriguing," said Taylor. "This unique adaptation brings up questions about the role of these abundant microbes in the ocean."

An under-microscope view shows clusters of cyanobacteria collected in the Pacific Ocean. Photo by Rachel Foster, University of California, Santa Cruz (UCSC)

Unlike all other known free-living cyanobacteria, this one lacks some of the genes needed to carry out photosynthesis, the process by which plants use light energy to make sugars out of carbon dioxide and water.

The mysterious microbe can do something very important, though: It provides natural fertilizer to the oceans by "fixing" nitrogen from the atmosphere into a form useable by other organisms.

"For it to have such an unusual metabolism is very exciting," Zehr said. "We're trying to understand how something like this can live and grow with so many missing parts."

Earlier research by Zehr's group had revealed surprisingly large numbers of novel nitrogen-fixing cyanobacteria, including the one that is the focus of this study, in the open ocean.

Although 80 percent of Earth's atmosphere is nitrogen, most organisms cannot use it unless it is "fixed" to other elements to make molecules like ammonia and nitrate. Because nitrogen is essential for all forms of life, nitrogen fixation is a major factor controlling overall biological productivity in the oceans.

The new microbe is one of the most abundant nitrogen fixers in many parts of the ocean, Zehr said.

New DNA sequencing technology provided by 454 Life Sciences enabled rapid sequencing of the organism's genome.

"I had begun to suspect that there was something missing in this organism's genome, and the genome sequencing confirmed that," said Zehr.

The results showed that it is missing the entire set of genes needed for photosystem II and carbon fixation, essential parts of the molecular machinery that carries out photosynthesis in plants and cyanobacteria.

"That has multiple implications," Zehr said. "It must have a 'lifestyle' that's very different from other cyanobacteria. Ecologically, it's important to understand its role in the ecosystem and how it affects the balance of carbon and nitrogen in the ocean."

During photosynthesis, photosystem II generates oxygen by splitting water molecules. Because oxygen inhibits nitrogen fixation, most nitrogen-fixing cyanobacteria only fix nitrogen at night, or do it in specialized cells. The lack of photosystem II enables the new microbe to fix nitrogen during the day, Zehr said.

But without photosynthesis, it can't take carbon dioxide from the atmosphere and convert it into sugars. So it's not clear how the new microbe feeds itself. Either it has some way of feeding on organic matter in its environment, or it lives in close association with other organisms that provide it with food, Zehr said.

"It would make a perfect symbiont because it could feed nitrogen to its host and live on the carbon provided by the host," he said.

Photosystem II is large complex of multiple proteins and chlorophyll molecules, but the team was unable to find any of the genes for the photosystem II core proteins. The genes for photosystem I appeared in the sequencing data, as did genes for both photosystems from the small numbers of contaminating cyanobacteria in the sample.

Zehr said he plans to continue research on the new microbe and fill some gaps in the present knowledge.

Marine scientists sample ocean waters for nitrogen fixing microorganisms in the South Pacific Ocean from the research vessel Kilo Moana. Photo by Jon Zehr, University of California, Santa Cruz (UCSC)

Efforts are currently underway to map the microbe's presence in the oceans and determine its global abundance.

Zehr is also interested in how its metabolism differs from other known cyanobacteria. If it can be cultured, there may be ways to exploit this organism's unusual metabolism in biotechnology applications, he said.

Zehr's coauthors on the paper include graduate student Shelley Bench, researcher Brandon Carter, and postdoctoral scholars Ian Hewson, Tuo Shi, and James Tripp of UCSC, as well as Faheem Niazi and Jason Affourtit of 454 Life Sciences.

The work was also supported by the Gordon and Betty Moore Foundation; sequencing was provided by 454 Life Sciences.

Contact:

Jonathan Zehr

Tel: (831) 459-4009

zehrj@ucsc.edu.

Article from the National Science Foundation (NSF) November 13,2008.